Seeding for mycoplasma - a very important analysis, it allows you not only to identify mycoplasma, define its appearance, but also can count the number of infectious agents, contained in 1 ml of the studied biological fluid. And it already solves the question of whether to prescribe this treatment to the patient.
Types of mycoplasmas that can be detected by means of sowing
Mikoplazmy - this is a very small negative microorganisms that cause infectious inflammation of urinary organs in men and women. Upon detection of mycoplasma is often found, and other pathogens of genital infection - Neisseria gonorrhoeae, Trichomonas vaginalis, Chlamydia
Chlamydia: insidious and widespread disease
and so on. All of these agents can also be identified during the bacteriological examination.
Seeding for mycoplasma properly called bacteriological examination. It carried out such a study in suspected mycoplasmosis urinary organs, when examining male and female infertility, pregnancy.
When sowing mycoplasma on nutrient media revealed two species of mycoplasmas related to the pathogenic microflora inhabiting the human sexual organs - Mycoplasma hominis and Ureaplasma urealitikum
Ureaplasma urealitikum - kind of mycoplasmas causing ureaplasmosis
. Also carried out to determine the sensitivity of microflora to antibiotics revealed.
There is another kind of Mycoplasma - Mycoplasma genitalium
Mycoplasma genitalium - most of the pathogenic mycoplasmas
Which most researchers attribute to the pathogenic microflora. But using this microorganism sowing can not be detected, because it grows in one - five months. To use it to identify other methods - PCR, ELISA, and others.
As the crop is taken for mycoplasma
The material for the study are usually smears from the urethra, vagina, cervix in women. Men - swabs from the urethra, prostate secretion, sperm, urine. Analysis taking before you start taking antibiotics. When taking a smear from the urethra collection of material is carried out before or no sooner than two hours after urination. It is impossible to analyze in women during menstruation - only in the period after the first week of the menstrual cycle before it is completed.
Since mycoplasma characterized by good adhesion (adhesion) to the epithelial cells, it is necessary to scrape the mucosa carefully to collect sufficient material for studies. The cell concentration in the material can influence the result.
As an excuse sowing
Seeding on mycoplasma today mostly carried out on special IST-environment for isolating and identifying mycoplasma, ureaplasma in biological material, as well as determining their amount in 1 ml of test material. The accuracy of the analysis is close to 100%.
Detection of mycoplasma is based on test systems MYCOPLASMA IST - 2, (BioMerieux, France), which is an abbreviated method to simultaneously detect mycoplasma, determine their type and content of semi-quantitative.
Test system 2 IST-sensitive in the presence of test material mycoplasma titer 1000 CFU / ml and above (cfu - colony forming unit is). This test system is used to detect mycoplasma. It allows you to get a viable culture of mycoplasmas by culture from the mucous membranes of sex organs. Wherein:
- It determines the type of mycoplasmas (Mycoplasma hominis and Ureaplasma
This insidious ureaplasma - often too late
- number of mycoplasmas (CFU, ie the degree of pathogenicity);
- Mycoplasma detection sensitivity to antibiotics - it allows the physician assigned to treat the most antibiotic that is exactly suppress the vital activity of the microorganisms.
Using test systems IST-2 can determine the sensitivity to the following antibiotics mycoplasma:
- tetracycline - tetracycline, doxycycline;
- macrolide - azithromycin (Sumamed), josamycin, erythromycin, clarithromycin, pristinomitsinu;
- fluoroquinolone antibiotics - ciprofloxacin, ofloxacin.
Evaluation of the results of research
If mycoplasma symptoms the patient is, the test material is usually determined by a low titer (less than 10,000 cfu / ml). Detection of Mycoplasma in the material of more than 10,000 CFU / ml indicates the presence of an infectious-inflammatory process.
Diagnostic tests play an important role in identifying diseases, sexually transmitted diseases. But the most important crops are still of the material on nutrient media - the study makes it possible not only to identify the infectious agent or Noah, but also to determine its quantity in the body of the patient, as well as to determine the sensitivity of detection of pathogen to antibiotics.