Seeding for mycoplasma - a very important analysis, it allows you not only to identify mycoplasma, define its appearance, but also can count the number of infectious agents, contained in 1 ml of the studied biological fluid. And it already solves the question of whether to prescribe this treatment to the patient.

Types of mycoplasmas that can be detected by means of sowing

Mikoplazmy - this is a very small negative microorganisms that cause infectious inflammation of urinary organs in men and women. Upon detection of mycoplasma is often found, and other pathogens of genital infection - Neisseria gonorrhoeae, Trichomonas vaginalis, Chlamydia Chlamydia: insidious and widespread disease   and so on. All of these agents can also be identified during the bacteriological examination.

Seeding for mycoplasma properly called bacteriological examination. It carried out such a study in suspected mycoplasmosis urinary organs, when examining male and female infertility, pregnancy.

When sowing mycoplasma on nutrient media revealed two species of mycoplasmas related to the pathogenic microflora inhabiting the human sexual organs - Mycoplasma hominis and Ureaplasma urealitikum Ureaplasma urealitikum - kind of mycoplasmas causing ureaplasmosis . Also carried out to determine the sensitivity of microflora to antibiotics revealed.

There is another kind of Mycoplasma - Mycoplasma genitalium Mycoplasma genitalium - most of the pathogenic mycoplasmas Which most researchers attribute to the pathogenic microflora. But using this microorganism sowing can not be detected, because it grows in one - five months. To use it to identify other methods - PCR, ELISA, and others.

As the crop is taken for mycoplasma

The material for the study are usually smears from the urethra, vagina, cervix in women. Men - swabs from the urethra, prostate secretion, sperm, urine. Analysis taking before you start taking antibiotics. When taking a smear from the urethra collection of material is carried out before or no sooner than two hours after urination. It is impossible to analyze in women during menstruation - only in the period after the first week of the menstrual cycle before it is completed.

Since mycoplasma characterized by good adhesion (adhesion) to the epithelial cells, it is necessary to scrape the mucosa carefully to collect sufficient material for studies. The cell concentration in the material can influence the result.

As an excuse sowing

Seeding on mycoplasma today mostly carried out on special IST-environment for isolating and identifying mycoplasma, ureaplasma in biological material, as well as determining their amount in 1 ml of test material. The accuracy of the analysis is close to 100%.

Detection of mycoplasma is based on test systems MYCOPLASMA IST - 2, (BioMerieux, France), which is an abbreviated method to simultaneously detect mycoplasma, determine their type and content of semi-quantitative.

Test system 2 IST-sensitive in the presence of test material mycoplasma titer 1000 CFU / ml and above (cfu - colony forming unit is). This test system is used to detect mycoplasma. It allows you to get a viable culture of mycoplasmas by culture from the mucous membranes of sex organs. Wherein:

• It determines the type of mycoplasmas (Mycoplasma hominis and Ureaplasma This insidious ureaplasma - often too late   urealitikum);
• number of mycoplasmas (CFU, ie the degree of pathogenicity);
• Mycoplasma detection sensitivity to antibiotics - it allows the physician assigned to treat the most antibiotic that is exactly suppress the vital activity of the microorganisms.

Using test systems IST-2 can determine the sensitivity to the following antibiotics mycoplasma:

• tetracycline - tetracycline, doxycycline;
• macrolide - azithromycin (Sumamed), josamycin, erythromycin, clarithromycin, pristinomitsinu;
• fluoroquinolone antibiotics - ciprofloxacin, ofloxacin.

Evaluation of the results of research

If mycoplasma symptoms the patient is, the test material is usually determined by a low titer (less than 10,000 cfu / ml). Detection of Mycoplasma in the material of more than 10,000 CFU / ml indicates the presence of an infectious-inflammatory process.

Diagnostic tests play an important role in identifying diseases, sexually transmitted diseases. But the most important crops are still of the material on nutrient media - the study makes it possible not only to identify the infectious agent or Noah, but also to determine its quantity in the body of the patient, as well as to determine the sensitivity of detection of pathogen to antibiotics.

Galina Romanenko

As is known, genital or genital herpes quite insidious disease, which is accompanied by periodic exacerbations. Depending on the frequency of recurrence of genital herpes, and determined the severity of the disease. When genital herpes in women Herpes in women - clinical features of the disease   treatment rather complex, long, and is mainly aimed at reducing the number of exacerbations and increase the "quiet" periods between them.

Every woman should remember that self-medicate genital herpes is invalid because, at best, it will not bring adequate relief, and in the worst case, will only aggravate the disease. Therefore, when the signs of the disease should seek medical help.

Principles of treatment of genital herpes in women

Treatment of genital herpes in women must pursue five objectives, which include:

• weaken or reduce the duration of symptoms such as itching, burning, pain, fever and an increase in regional lymph nodes;
• reduce the period of complete healing of herpes sores;
• reduce the duration and severity of viral places herpetic lesions;
• reduce the frequency and severity of exacerbations of the disease;
• eliminate (remove) the infection in order to prevent relapse.

Medication

The scheme treatment of genital herpes Genital herpes - long treatment   in women include the use of etiotropic (antiviral) and immune-boosting drugs. Treatment of genital herpes can be divided into three phases.

• Phase one (exacerbation)

In this phase included the appointment of antiviral drugs, such as alpirazin at 0, 1 gram five times a day for five days or Zovirax Zovirax - time-proven antiviral drug   (acyclovir viroleks) 200 mg five times a day for five days. Then, the drug cut up to four times a day, which should continue for two to three weeks. Additionally assigned ascorbic acid 1 gram twice daily for fifteen days. You must also be administered intramuscularly antiherpetic immunoglobulin of 3 ml every three to seven days, which is the entire course of five injections. Maybe replace antiherpetic measles immunoglobulin immunoglobulin 3 ml intramuscularly once in three days, a total of four injections.

To stimulate immunity Immunity - types and characteristics in children in adults   subcutaneously with 1 0 mL twice a week administered taktivin, treatment is ten injections. As a topical treatment applied ointment "gossypol" "Megasin" "bonafton" or "Alpirazin" (for the treatment of vaginal four or five times a day).

• Phase Two (stihanie relapse)

In this phase included the appointment of vitamins B1, B6 a day one ml, the whole course of fifteen injections. Additionally, 10% is assigned to the calcium chloride one tablespoon three times a day for three weeks or calcium gluconate at 0, 5 grams three times a day (the rate of ten to fifteen days). Shown autohaemotherapy starting from 2 ml to 10 ml to 10 ml to 2 ml. Tazepama possible to use (for normalization of sleep), one tablet twice a day for three weeks, and tavegila (as antiallergic agent) one tablet twice a day for three weeks. As immunostimulants can be administered by infusion Eleutherococcus Dibazolum 20 drops and one tablet twice daily (treatment for three weeks). Ongoing local treatment of the above ointments.

• Phase three (remission)

This phase is carried out vaccinotherapy herpes vaccine, the use of which provides a relapse for at least two months. Vaccine is carried out only after the course of a restorative treatment and symptoms of the disease subsided. The vaccine is injected intradermally (inner side of the forearm) at 0, 3 ml once in three days, the whole course of treatment was five injections. Then, after a two-week break conducted five injections at 0, 3 ml once every seven days. If there were herpes, may increase the intervals between injections twice. Six months later, a second course, just as there should be four to six courses.

Anna Sozinova